COVID-19: biomarkers linked to severe forms of the disease

Above all, this is the result of a tremendous collective effort by several dozen people involved in research and clinical work at the CHUM, who participated in this study and in the Quebec COVID-19 (BQC19) Biobank.

Based on a blood test, we were able to inventory the immune-cell populations present in 50 patients with SARS-CoV-2 and compare them to those of 22 patients of similar gender and age hospitalized for other acute illnesses, and those of 49 healthy controls.

This immune profiling allowed us to identify subsets of “dysregulated” immune cells specific to patients affected by SARS-CoV-2. Most notably, some of these immune alterations were associated with ventilation needs and mortality in these same patients.

These markers specific to SARS-CoV-2 could then help us to identify the patients at greatest risk, and suggest new avenues for developing therapeutic targets.

In addition, we confirm what has been observed in other studies: disturbances in the immune system such as neutrophilia or lymphopenia, for example, are related to the severity of the disease in hospitalized patients, but are not specific to SARS-CoV-2.

From a clinical perspective, this could explain why general anti-inflammatory treatments such as steroids seem to work in COVID-19 and other acute diseases.

These assays could be easily transferred to clinical settings, because we worked with very small amounts of blood– less than one millilitre –to develop these immune profiles. 

In our experimental approach, we used techniques commonly employed in hospital laboratories: using surface antibodies to “stain” the cells that express certain markers, flow cytometry (cell characterization and counts), etc. In a clinical setting, this could help track the progress of patients over time and ultimately identify those at high risk who would require closer monitoring.

I hope that our study will lead to the development of biomarkers that will help us stratify patients based on their risk of developing a severe form of the disease. This should also allow us to identify new therapeutic targets, and to better select those patients who might benefit from certain available therapeutic approaches.